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Interdisciplinary studies of DNA-protein interaction using single molecule fluorescence and nano-fabrication
 

Time: 2月26日下午2:00-3:00 pm

Date: Feb. 26th(Tue.), 2013
Location: The Institute for Biomedical Engineering & Nano Science (iNANO), 67 Chifeng Road
Reporter: 王锋 博士(Associate Research Scientist,Department of Biochemistry and Molecular Biophysics,Columbia University,USA)
Abstract:
Single molecule study and nano-fabrication are two emerging fields in recent years. They have brought us numerous discoveries and applications. We integrate these two techniques and invented the “DNA curtain” and its variations. Using “DNA curtain” and single molecule fluorescence, I have studied the promoter search mechanism of RNA polymerase and molecular pathway of DNA mismatch. I found that promoter search by RNA polymerase is dominated by 3D diffusion and protein concentration plays a direct role in indicating the search pathway. And I also discovered that the diffusion and function of mismatch proteins are regulated along the mismatch pathway. In the future, I propose an interagrated study of genomics and epigenomics based on nano-fabrication with the goal to develop a high-throughput, high-resolution and inexpensive technology to detect DNA structure variation and methylation simultaneously.
主持:林超 博士
 
 

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