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Safety Training and the 4th SEMINAR of iNANO
13:30--13:50 Safety Training
13:50--14:20 SEMINAR 1
Speaker:Meng Chu(褚萌)
Title: Synthesis and Application of Polycationic Gene Vectors with nuclear targeting ligand
Abstract:Gene therapy essentially consists of introducing specific genetic material into target cells without producing toxic effects on surrounding tissue. A major obstacle in successful gene therapy is the lack of a suitable gene delivery vector. It is hypothesized that a remarkable gene vector should have the ability to perform multiple functions including stability, biocompatibility, biodegradability, targetability and efficient intracellular trafficking toward the cell nucleus. Among various gene vectors, the non-viral polycations have attracted great interests for their unique properties including convenience of the production, adaptability of the structures, high safety and high stability. However, inefficient endosome release, cytoplasmic transport and nuclear entry of plasmids have currently become the bottlenecks of further applications. Besides, transfection is usually concomitant with concerns of cytotoxicity or biosafety, the efficiency of transfection also varies with different gene vector and cell type. Hence, it is important to design novel cationic copolymers with low cytotoxicity and high transfection efficiency for gene therapy .
14:20--14:50 SEMINAR 2
Speaker:Lingling Dai(戴玲玲)
Title:In vitro study of SPIO- labeled human pancreatic cancer cell line BxPC-3(Tong M, Xiong F, Shi Y, et al.Contrast media & molecular
Abstruct:The survivin gene is highly expressed in pancreatic cancer. The purpose of this study was to design and synthesize functionalized magnetic iron oxide nanoparticles (MNPs) targeting survivin gene for the detection of pancreatic cancer. The pancreatic cancer cell line BxPC-3 with survivin gene expression was selected in this study.The healthy lung fibroblast cell was used as a control. Chitosan-coated MNPs (CS@MNPs) and antisense oligode oxynu-cleotide of survivin gene were conjugated to MNPs to give Sur-MNPs. Fourier transform infrared spectroscopy was performed to confirm the conjunction of chitosan. The interactions of MNPs, CS@MNPs, and Sur-MNPs in BxPC-3 cells were observed, recorded and analyzed. The size, morphology, cell uptake, cytotoxicity and stability of those particles were assessed by transmission electron microscope, Prussian blue staining, MTT assay and agarose gel electrophoresis. The magnetic resonance signal intensities of pancreatic cells labeled with CS@MNPs and MNPs, and Sur-MNPs, were compared on T2-weighted images. The results demonstrated that the level of cellular uptake of CS@MNPs was higher than that of naked MNPs. The Sur-MNPs had a suitable size (12 nm sized core), high stability, no cytotoxicity and good water dispersion. Sur-MNPs did not accumulate in healthy lung fibroblast cells, while being taken up by BxPC-3 cells .The Sur-MNPs in BxPC-3 cells could be visualized on T2-weighted images, which suggested that Sur-MNPs could be used to detect the expression of survivin gene. Thus, Sur-MNPs may be a potential molecular imaging probe targeting survivin gene for early detection of pancreatic cancer cells.
Magnetic Nanoparticles (MNPs)Synthesis and Characterization
Location: Meeting Room 219
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People Home Research Facilities Student News Seminar Yao Qin, Ph.D. Bingbo Zhang, Ph.D.